| # |
GPM |
# proteins |
Description |
|
1. |
GPM10100154812
peptide
model
gel
aaa
go
mh
|
1318 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0000671
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Kidney crude preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
2. |
GPM10100154813
peptide
model
gel
aaa
go
mh
|
863 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0000671
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Kidney pure preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
3. |
GPM10100154814
peptide
model
gel
aaa
go
mh
|
1092 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0000759
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Liver crude preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
4. |
GPM10100154815
peptide
model
gel
aaa
go
mh
|
890 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0000759
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Liver pure preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
5. |
GPM10100154816
peptide
model
gel
aaa
go
mh
|
1705 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0001078
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Placental crude preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
6. |
GPM10100154817
peptide
model
gel
aaa
go
mh
|
1061 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0001078
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Placental pure preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
7. |
GPM10100154818
peptide
model
gel
aaa
go
mh
|
1320 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0000146
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Brian stem crude preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
8. |
GPM10100154819
peptide
model
gel
aaa
go
mh
|
814 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0000146
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Brian stem pure preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
9. |
GPM10100154820
peptide
model
gel
aaa
go
mh
|
1507 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0000233
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Cerebrum crude preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
10. |
GPM10100154821
peptide
model
gel
aaa
go
mh
|
783 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0000233
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Cerebrum pure preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
11. |
GPM10100154822
peptide
model
gel
aaa
go
mh
|
712 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0001103
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Skeletal muscle crude preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
12. |
GPM10100154823
peptide
model
gel
aaa
go
mh
|
448 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0001103
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Skeletal muscle pure preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
13. |
GPM10100154824
peptide
model
gel
aaa
go
mh
|
1212 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0001279
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Spinal cord crude preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
14. |
GPM10100154825
peptide
model
gel
aaa
go
mh
|
829 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0001279
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Spinal cord pure preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
15. |
GPM10100154826
peptide
model
gel
aaa
go
mh
|
736 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0000562
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Heart crude preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
16. |
GPM10100154827
peptide
model
gel
aaa
go
mh
|
570 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0000562
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Heart pure preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
17. |
GPM10100154828
peptide
model
gel
aaa
go
mh
|
2204 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0001363
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Testis crude preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
18. |
GPM10100154829
peptide
model
gel
aaa
go
mh
|
1404 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0001363
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Testis pure preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
19. |
GPM10100154830
peptide
model
gel
aaa
go
mh
|
996 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0000232
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Cerebellum crude preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
|
|
20. |
GPM10100154831
peptide
model
gel
aaa
go
mh
|
922 |
- BRENDA cell culture: none
- BRENDA tissue: BTO:0000232
 - CELL cell type: none
- GO subcellular: GO:0005739
 - email: scarr@broad.edu
- institution: Broad Institute
- name: Steven A. Carr
- project: A mitochondrial protein compendium elucidates complex I disease biology: subtractive
- project comment: Cerebellum crude preparation. Tranche
Pagliarini, DJ, Calvo, SE, Chang, B, Sheth, SA, Vafai, SB, Ong, SE, Walford, GA, Sugiana, C, Boneh, A, Chen, WK, Hill, DE, Vidal, M, Evans, JG, Thorburn, DR, Carr, SA and Mootha, VK, Cell, 2008, 134, 112-123. Crude and pure mitochondria from 10 mouse tissues were purified and in-solution digested with trypsin following reduction/alkylation of cysteines with iodoacetamide. LC-MS/MS was performed on an LTQ-Orbitrap. Four technical replicate LC-MS/MS runs from a single preparation were performed for the crude and pure cerebellum samples. Single runs were performed for the other 9 tissues (cerebrum, brainstem, spinal cord, kidney, liver, heart, skeletal muscle, testis and placenta). Instrument: Thermo Fisher Scientific, LTQ-Orbitrap, nESI.
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Page 1 of 2 | 1 | 2 | Key word(s): mitochondrial protein compendium
Total protein ids = 25864
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